Anti-proliferative, antioxidant effects of methanol extract of Calotropis procera leaf on lung cancer cells (H1299) and its ameliorative effect on expression of CD146 on blood cells

Abstract Background Calotropis procera leaf is one of the plants commonly utilized in phytomedicine Nigeria. The present investigation explored use extracts on cell viability and apoptosis respectively. In this study, expression Cluster differentiation 146 (CD146) blood lung cancer patients regulatory T cells (Tregs) was determined. antioxidant anti-proliferative effects methanol Caloropis H1299 were investigated. Methods From flow cytometry, CD146+ evaluated using healthy patient, adenocarcinoma, squamous, small granulocytes, monocytes, lymphocytes, CD4+, Treg determined by 7-amino-actinomycin D/Annexin V-Allophycocyanin (APC) staining during resting stage after 24 h Immunofluorescence conducted. Cell assay, hydroxyl (OH), hydrogen peroxide (H 2 O ) nitric oxide (NO) scavenging radicals Reducing power flavonoid content effect at different concentrations 24hrs Results includes 4.60 % patients, 10.10, 12.20, 9.80 squamous cancer. CD4+ V-APC which indicate that also occurred Treg. shows presence CD146 patient’s tissues. have effects. Methanol extract reduced h. Conclusion Increased indicates it may be a possible target for treatment utilizing potent immunotherapy or natural products such as ameliorate CD146. has antioxidant, inhibitory capacity cells, ability to scavenge OH, H 2, NO radicals. Hence, strengthens phyto-medicinal properties procera.